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The assembly pathway of the mitochondrial carrier translocase involves four preprotein translocases.| Autors: | Karina Wagner, Natalia Gebert, Bernard Guiard, Katrin Brandner, Kaye N Truscott, Nils Wiedemann, Nikolaus Pfanner, Peter Rehling | | Idioma: | Eng. | | Data: | 16-06-2008 | | Revista: | Molecular and cellular biology
(1098-5549)
| | Lliurament: | Mol Cell Biol. 2008 Jul;28(13):4251-60 |
Abstract:
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The mitochondrial inner membrane contains preprotein translocases that mediate insertion of hydrophobic proteins. Little is known about how the individual components of these inner membrane preprotein translocases combine to form multisubunit complexes. We have analyzed the assembly pathway of the three membrane-integral subunits Tim18, Tim22, and Tim54 of the twin-pore carrier translocase. Tim54 displayed the most complex pathway involving four preprotein translocases. The precursor is translocated across the intermembrane space in a supercomplex of outer and inner membrane translocases. The TIM10 complex, which translocates the precursor of Tim22 through the intermembrane space, functions in a new posttranslocational manner: in case of Tim54, it is required for the integration of Tim54 into the carrier translocase. Tim18, the function of which has been unknown so far, stimulates integration of Tim54 into the carrier translocase. We show that the carrier translocase is built via a modular process and that each subunit follows a different assembly route. Membrane insertion and assembly into the oligomeric complex are uncoupled for each precursor protein. We propose that the mitochondrial assembly machinery has adapted to the needs of each membrane-integral subunit and that the uncoupling of translocation and oligomerization is an important principle to ensure continuous import and assembly of protein complexes in a highly active membrane.
| | Copyright: | Molecular and cellular biology
Institut für Biochemie und Molekularbiologie, ZBMZ, Universität Freiburg, D-79104 Freiburg, Germany. | | Full text: |  EBSCO - HTML (amb subscripció) | | | |
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