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An endocytic mechanism for haemoglobin-iron acquisition in Candida albicans.| Autors: | Ziva Weissman, Revital Shemer, Elizabeth Conibear, Daniel Kornitzer | | Idioma: | Eng. | | Data: | 30-06-2008 | | Revista: | Molecular microbiology
(1365-2958)
| | Lliurament: | Mol Microbiol. 2008 Jul;69(1):201-17 |
Abstract:
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The fungal pathogen Candida albicans is able to utilize haemin and haemoglobin as iron sources. Haem-iron utilization is facilitated by Rbt5, an extracellular, glycosylphophatidylinositol (GPI)-anchored, haemin- and haemoglobin-binding protein. Here, we show that Rbt5 and its close homologue Rbt51 are short-lived plasma membrane proteins, degradation of which depends on vacuolar activity. Rbt5 facilitates the rapid endocytosis of haemoglobin into the C. albicans vacuole. We relied on recapitulation of the Rbt51-dependent haem-iron utilization in Saccharomyces cerevisiae to identify mutants defective in haemoglobin utilization. Homologues of representative mutants in S. cerevisiae were deleted in C. albicans and tested for haemoglobin-iron utilization and haemoglobin uptake. These mutants define a novel endocytosis-mediated haemoglobin utilization mechanism that depends on acidification of the lumen of the late secretory pathway, on a type I myosin and on the activity of the ESCRT pathway.
| | Copyright: | Molecular microbiology
Department of Molecular Microbiology, Technion-B. Rappaport Faculty of Medicine, and the Rappaport Institute for Research in the Medical Sciences, Haifa 31096, Israel. | | Full text: |  EBSCO - HTML (amb subscripció) | | | |
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