Immunological investigation of the hepatic tissue from infants with biliary atresia.

Abstract

PURPOSE:
Matrix metalloproteinases (MMPs) and their endogenous tissue inhibitors [tissue inhibitors of metalloproteinases (TIMPs)] have been implicated in tissue injury and remodeling in many organs.

The objective of this study was to evaluate the expression of MMP-3 and -9, and TIMP-1, -2, and -3 and their relationship to liver fibrosis in infants with biliary atresia.

METHODS:
The expression of MMP-3 and-9 and TIMP-1, -2 and -3 was investigated in liver tissue samples of nine patients with biliary atresia.

In addition, the expression of CCR-4 and CCR-5 was analyzed to investigate the activation of Th1 and Th2 cells.

The mRNA levels were measured by semiquantitative reverse transcriptase polymerase chain reaction.

RESULTS:
The expression of MMP-3 was higher than that of MMP-9 in all samples (P < 0.01). The expression of TIMP-1 was higher than that of TIMP-2 and -3 in all samples (P < 0.01). The expression of CCR-5 was higher than that of CCR-4 (P < 0.05), which implied higher activation of Th1 cells relative to Th2 cells.

CONCLUSION:
Our findings suggest that MMP-3, possibly induced by Th1 cytokines, and its balance with TIMP-1, may be one of the factors involved in the pathogenesis of biliary atresia.

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Authors

• Haruna Baba
• Yoshikazu Ohtsuka
• Tohru Fujii
• Hidenori Haruna
• Satoru Nagata
• Hiroyuki Kobayashi
• Atsuyuki Yamataka
• Toshiaki Shimizu
• Takeshi Miyano
• Yuichiro Yamashiro

Publication date

2009-01-23

Journal

Journal topics

• General Surgery
• Pediatrics

Language

Eng.

Copyright

Pediatric Surgery International

Department of Pediatrics and Adolescence Medicine, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.

Release reference

Pediatr Surg Int. 2009 Feb;25(2):157-62

Related books

• Books of General Surgery
• Books of Pediatrics