A functional genomic screen reveals novel host genes that mediate interferon-alpha's effects against hepatitis C virus.

Abstract

We identified 93 genes that mediate the anti-HCV effect of IFN-α through genome-wide siRNA screening; 23 and nine genes were involved in mRNA processing and translation initiation, respectively.

These findings reveal an unexpected role for mRNA processing in generation of the antiviral state, and suggest a new avenue for therapeutic development in HCV.

Full Text

• DOI - Journal of Hepatology (DOI)
• Swets Information Services - full-text online
• OhioLINK Electronic Journal Center - full-text online
• Elsevier Science - full-text online
• EBSCO - full-text online

Subjects

• Antigens, Neoplasm
• Antiviral Agents
• Cell Line
• Computational Biology
• Genome, Human
• Genomics
• Hepacivirus
• Hepatitis C, Chronic
• High-Throughput Screening Assays
• Host-Pathogen Interactions
• Humans
• Interferon-alpha
• Pharmacogenetics
• RNA, Small Interfering
• Receptor, Interferon alpha-beta
• Replicon
• Ribonucleoproteins, Small Nuclear

Similar articles

• HCV infection induces inflammatory cytokines and chemokines mediated by the cross-talk between hepatocytes and stellate cells. (2013-05-15)
• A Genetic Screen Identifies Interferon Alfa Effector Genes Required to Suppress Hepatitis C Virus Replication. (2013-02-24)
• Molecular architecture of the human pre-mRNA 3' processing complex. (2009-02-16)
• Genome-wide analysis of host mRNA translation during hepatitis C virus infection. (2013-04-03)
• Altered of apoptotic markers of both extrinsic and intrinsic pathways induced by hepatitis C virus infection in peripheral blood mononuclear cells. (2012-12-20)
• Hepatitis C virus core protein induces hepatic metabolism disorders through down-regulation of the SIRT1-AMPK signaling pathway. (2013-03-16)
• Transcriptional Control by NF-κB: Elongation in Focus. (2013-04-25)
• HCV IRES-mediated core expression in zebrafish. (2013-03-01)
• IFITM1 is a tight junction protein that inhibits hepatitis C virus entry. (2012-12-12)

Authors

• Hong Zhao
• Wenyu Lin
• Kattareeya Kumthip
• Du Cheng
• Dahlene N Fusco
• Oliver Hofmann
• Nikolaus Jilg
• Andrew W Tai
• Kaku Goto
• Leiliang Zhang
• Winston Hide
• Jae Young Jang
• Lee F Peng
• Raymond T Chung

Publication date

2012-01-18

Journal

Journal topics

• Liver
• Liver Diseases

Language

Eng.

Copyright

Journal of Hepatology

Department of Infectious Diseases, Peking University First Hospital, Beijing 100034, China.

Release reference

J Hepatol. 2012 Feb;56(2):326-33

Related books

• Books of Antigens, Neoplasm
• Books of Antiviral Agents
• Books of Cell Line
• Books of Computational Biology
• Books of Genome, Human
• Books of Genomics
• Books of Hepacivirus
• Books of Hepatitis C, Chronic
• Books of High-Throughput Screening Assays
• Books of Host-Pathogen Interactions
• Books of Interferon-alpha
• Books of Liver
• Books of Liver Diseases
• Books of Pharmacogenetics
• Books of RNA, Small Interfering
• Books of Receptor, Interferon alpha-beta
• Books of Replicon
• Books of Ribonucleoproteins, Small Nuclear