We have developed a novel microsphere-based genotyping method for 46 mucosal human papillomavirus (HPV) types. HPV DNA was amplified by PCR using general primers and typed by hybridization to HPV type-specific probes coupled to sortable microspheres based on the Luminex xMAP technology.
Hybridization to each probe was specific for each HPV type without cross-hybridization and sensitive enough to allow typing of HPV contained in clinical specimens.
The method was validated with direct sequencing and the Roche Linear Array genotyping method.
2012-01-23
Eng.
Journal of clinical microbiology
National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
J Clin Microbiol. 2012 Feb;50(2):460-4
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