Tumorigenic cells are common in mouse MPNSTs but their frequency depends upon tumor genotype and assay conditions.

Abstract

Tumor-initiating cells have been suggested to be rare in many cancers.

We tested this in mouse malignant peripheral nerve sheath tumors (MPNSTs) and found that 18% of primary and 49% of passaged MPNST cells from Nf1(+/-); Ink4a/Arf(-/-) mice formed tumors upon transplantation, whereas only 1.8% to 2.6% of MPNST cells from Nf1(+/-); p53(+/-) mice did. MPNST cells of both genotypes require laminin binding to β1-integrin for clonogenic growth.

Most MPNST cells from Nf1(+/-); Ink4a/Arf(-/-) mice expressed laminin, whereas most MPNST cells from Nf1(+/-); p53(+/-) mice did not.

Exogenous laminin increased the percentage of MPNST cells from Nf1(+/-); p53(+/-) but not Nf1(+/-); Ink4a/Arf(-/-) mice that formed tumorigenic colonies. Tumor-forming potential is common among MPNST cells, but the assay conditions required to detect it vary with tumor genotype.

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Subjects

• Animals
• Antigens, CD29
• Cell Proliferation
• Cell Transformation, Neoplastic
• Genotype
• Laminin
• Mice
• Nerve Sheath Neoplasms
• Tumor Cells, Cultured

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Authors

• Johanna Buchstaller
• Paul E McKeever
• Sean J Morrison

Publication date

2012-02-20

Journal

Journal topics

• Neoplasms

Language

Eng.

Copyright

Department of Internal Medicine, Life Sciences Institute, and Center for Stem Cell Biology, University of Michigan, Ann Arbor, MI 48109-2216, USA.

Release reference

Cancer Cell. 2012 Feb;21(2):240-52

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