Viability and function of the cryopreserved whole rat ovary: comparison between slow-freezing and vitrification.

Abstract

We have developed a new method that can be used in basic studies to improve cryopreservation protocols.

Our initial findings suggest that a moderate concentration of the cryoprotectant DMSO is superior to a high DMSO concentration for both vitrification and slow freezing.

Full Text

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Subjects

• Animals
• Apoptosis
• Caspase 3
• Cryopreservation
• Cryoprotective Agents
• Dimethyl Sulfoxide
• Dose-Response Relationship, Drug
• Estradiol
• Female
• Fertility Preservation
• Freezing
• Immunohistochemistry
• Organ Preservation
• Ovarian Follicle
• Ovary
• Perfusion
• Rats
• Rats, Sprague-Dawley
• Time Factors
• Tissue Culture Techniques
• Tissue Survival
• Vitrification

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Authors

• Milan Milenkovic
• César Diaz-Garcia
• Ann Wallin
• Mats Brännström

Publication date

2012-04-30

Journal

Journal topics

• Fertility
• Infertility

Language

Eng.

Copyright

Fertility and sterility

Department of Obstetrics and Gynecology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Release reference

Fertil Steril. 2012 May;97(5):1176-82

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